Diploma subjects

Lecture - Biological activity and synthesis of glycopeptides and their precursors

Characteristics of amino acids and sugars, synthesis of peptides, glycoproteins and simple sugar compounds, characteristics of methods for the purification and identification of biomolecules (chromatography, electrophoresis, IR spectroscopy, UV-VIS, NMR, mass spectrometry), the role and functions of peptides, proteins, sugars and glycoproteins in the body, characteristics of selected peptides and sugars.

The seminar will be divided into two parts. The first of them will be presentations prepared by students and showing theoretical issues that are an extension of the lecture.
In the second part of the seminar, graduate students will present the results of experimental research constituting their diploma projects.

Experimental diploma projects:

1. Obtaining protected amino acid derivatives.
2. Peptide synthesis using SPPS (Solid Phase Peptide Synthesis).
3. Analysis of the mixture of amino acids using chromatographic techniques (TLC, HPLC).
4. Identification of a mixture of biomolecules based on spectroscopic methods.
5. Synthesis of selected sugar compounds which are precursors of glycopeptides.
6. Analysis of polysaccharides by gel electrophoresis.
7. Analysis and purification of peptides based on HPLC and MS.
8. Analysis of peptides and proteins based on sequencing.
9. Influence of the glycosyl donor on the efficiency of the reaction of the formation of serine glycosides.
10. Synthesis of simple sugar compounds for the synthesis of muramyl peptides.
11. The use of chromatography for the separation of sugar compounds.